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the fast & easy way to design your primer
» Calculate annealing temperature of your primer.
primer sequence:
» Calculate annealing temperature of your primer pair.
primer sequence 1:
primer sequence 2:
» Calculate the number of A or T nucleotides necessary to accomplish the designated annealing temperature.
primer length [bp]
designated melting
temperature [°C]:
neccessary
A/T nucleotides
» Change your sequence to complementary and reverse.
sequence:
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