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the fast & easy way to design your primer
Insert your nucleotide sequence (FASTA or plain text):
>enolase of E. coli K12 ATGTCCAAAATCGTAAAAATCATCGGTCGTGAAATCATCGACTCCCGTGGTAACCCGACT GTTGAAGCCGAAGTACATCTGGAGGGTGGTTTCGTCGGTATGGCAGCTGCTCCGTCAGGT GCTTCTACTGGTTCCCGTGAAGCTCTGGAACTGCGCGATGGCGACAAATCCCGTTTCCTG GGTAAAGGCGTAACCAAAGCTGTTGCTGCGGTAAACGGCCCGATCGCTCAGGCGCTGATT GGCAAAGATGCTAAAGATCAGGCTGGCATTGACAAGATCATGATCGACCTGGACGGCACC GAAAACAAATCCAAATTCGGCGCGAACGCAATCCTGGCTGTATCTCTGGCTAACGCCAAA GCTGCTGCAGCTGCTAAAGGTATGCCGCTGTACGAGCACATCGCTGAACTGAACGGTACT CCGGGCAAATACTCTATGCCGGTTCCGATGATGAACATCATCAACGGTGGTGAGCACGCT GACAACAACGTTGATATCCAGGAATTCATGATTCAGCCGGTTGGCGCGAAAACTGTGAAA GAAGCCATCCGCATGGGTTCTGAAGTTTTCCATCACCTGGCAAAAGTTCTGAAAGCGAAA GGCATGAACACTGCTGTTGGTGACGAAGGTGGCTATGCGCCGAACCTGGGTTCCAACGCT GAAGCTCTGGCTGTTATCGCTGAAGCTGTTAAAGCTGCTGGTTATGAACTGGGCAAAGAC ATCACTTTGGCGATGGACTGCGCAGCTTCTGAATTCTACAAAGATGGTAAATACGTTCTG GCTGGCGAAGGCAACAAAGCGTTCACCTCTGAAGAATTCACTCACTTCCTGGAAGAACTG ACCAAACAGTACCCGATCGTTTCTATCGAAGACGGTCTGGACGAATCTGACTGGGACGGT TTCGCATACCAGACCAAAGTTCTGGGCGACAAAATCCAGCTGGTTGGTGACGACCTGTTC GTAACCAACACCAAGATCCTGAAAGAAGGTATCGAAAAAGGTATCGCTAACTCCATCCTG ATCAAATTCAACCAGATCGGTTCTCTGACCGAAACTCTGGCTGCAATCAAGATGGCGAAA GATGCTGGCTACACTGCAGTTATCTCTCACCGTTCTGGCGAAACTGAAGACGCTACCATC GCTGACCTGGCTGTTGGTACTGCTGCAGGCCAGATCAAAACTGGTTCTATGAGCCGTTCT GACCGTGTTGCTAAATACAACCAGCTGATTCGTATCGAAGAAGCTCTGGGCGAAAAAGCA CCGTACAACGGTCGTAAAGAGATCAAAGGCCAGGCATAA
primer length:
bp
A/T nucleotides:
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3' G/C:
opt. product size:
bp
Poly-X-Protection:
